Canine VEGF Quantikine ELISA Kit

返回产品中心

Canine VEGF Quantikine ELISA Kit

深圳子科生物优势代理美国R&DELISA系列产品试剂盒，价格优势，货期短，欢迎来电咨询0755-33164177！

Canine VEGF Quantikine ELISA Kit Summary

货号：CAVE00 规格:96T

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (200 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)

Sensitivity

19.5 pg/mL

Assay Range

19.5 - 1,250 pg/mL (Cell Culture Supernates), 39.1 - 2,500 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant canine VEGF. This assay cross-reacts with Human VEGF165.

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity observed with 1 or more species tested.

Interference

Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Canine VEGF Immunoassay is a 4.5 hour solid phase ELISA designed to measure VEGF levels in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant canine VEGF and antibodies raised against the recombinant protein. Results obtained for naturally occurring canine VEGF showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural canine VEGF.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	29.1	123	531	32.8	128	495
Standard Deviation	1.9	5	18.4	2.8	6.4	33
CV%	6.5	4.1	3.5	8.5	5	6.7

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	53.7	235	910	64.5	250	1003
Standard Deviation	3.6	10.6	46.2	5.7	17.4	61.7
CV%	6.7	4.5	5.1	8.8	7	6.2

Recovery

The recovery of canine VEGF spiked to three different levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	102	94-110
EDTA Plasma (n=4)	98	92-107
Heparin Plasma (n=4)	107	96-118
Serum (n=4)	103	87-113

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of canine VEGF and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Mouse Chemerin Quantikine ELISA Kit