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Human Cystatin B Quantikine ELISA Kit

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Human Cystatin B Quantikine ELISA Kit

Catalog #	Availability	Size / Price
DCYB00	Contact your distributor for availability.	1 Kit / Please Inquire

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (17 uL), EDTA Plasma (17 uL), Heparin Plasma (17 uL), Saliva (10 uL), Urine (13 uL), Human Milk (10 uL)

Sensitivity

0.034 ng/mL

Assay Range

0.2 - 10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)

Specificity

Natural and recombinant human Cystatin B

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Cystatin B Immunoassay is a 4.5 hour solid phase ELISA designed to measure Cystatin B levels in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains E. coli-expressed recombinant human Cystatin B and antibodies raised against the recombinant protein. Results obtained for naturally occurring human Cystatin B and recombinant human Cystatin B showed linear curves that were parallel to the standard curves obtained using the Quantikine Human Cystatin B Immunoassay standards. These results indicate that this kit can be used to determine relative mass values for natural human Cystatin B.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	1.38	2.73	5.47	1.42	2.78	5.4
Standard Deviation	0.049	0.082	0.164	0.148	0.191	0.263
CV%	3.6	3	3	10.4	6.9	4.9

Recovery

The recovery of Cystatin B spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	102	90-115
EDTA Plasma (n=4)	96	86-103
Heparin Plasma (n=4)	90	81-97
Serum (n=4)	93	83-103

Linearity

To assess the linearity of the assay, samples containing high concentrations of Cystatin B were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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